在我国,胆红素含量测定的基本原理是利用胆红素与重氮化的对-氨基苯磺酸发生偶联反应生成偶氮染料,这种偶氮染料在酸性介质中呈色,测定其吸光度计算胆红素含量。但是我们发现在夏季用本法测定结果重现性较差,于是对这种染料在乙醇和甲醇中最大吸收峰波长和稳定性作了初步考察,以便为更好的控制胆汁和胆红素及有关制剂质量提供考参。 In our country, the basic principle of bilirubin determination is the use of bilirubin and diazotized para-aminobenzenesulfonic acid coupling reaction generated azo dyes, the azo dyes in acidic medium color, its Absorbance calculated bilirubin content. However, we found that the results of this method were poorly reproducible in the summer, and preliminary investigation was made on the wavelength and stability of the maximum absorption peak of this dye in ethanol and methanol in order to better control bile and bilirubin and The quality of preparations to provide test parameters.