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报道了含△６去饱和酶的深黄被孢霉菌丝提取物催化亚油酸合成γ－亚麻酸．研究了辅酶、温度、时间等对γ－亚麻酸合成的影响．结果表明,反应温度降低,γ－亚麻酸的产率提高．在１０℃以下．γ－亚麻酸的产率达到最大值;苹果酸盐对该反应有明显促进作用．γ－亚麻酸的产率随体系ｐＨ增加而增加,且在ｐＨ＝７~８时产率较高;该反应需在分子氧存在下进行;ＮＡＤＰＨ,ＡＴＰ和ＣｏＡ是该反应的必需辅酶．在优化的反应条件下,γ－亚麻酸的产量达到０．２１ｍｇ/ｍＬ。
It has been reported that the Mycelia flavum hyphae extract with Δ6 desaturase catalyzes the synthesis of γ-linolenic acid by linoleic acid. The effects of coenzyme, temperature and time on the synthesis of γ-linolenic acid were studied. The results showed that the reaction temperature decreased, γ-linolenic acid yield increased. Below 10 ℃. The yield of γ-linolenic acid reached its maximum; malate significantly promoted the reaction. The yield of γ-linolenic acid increased with increasing pH of the system, and was higher at pH = 7-8. The reaction was performed in the presence of molecular oxygen. NADPH, ATP and CoA were necessary coenzymes for the reaction. Under optimized reaction conditions, the yield of γ-linolenic acid reached 0.21 mg / mL.