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为了观察产前束缚应激对子代大鼠空间学习记忆能力、海马神经干细胞增殖及巢蛋白表达的影响,将体重240~260 g的Sprague-Dawley雌性母鼠12只随机分成2组,对照组于孕期不做任何处理,束缚应激组于孕14~20 d时给予束缚应激,3次/天,45 min/次。取1月龄子代大鼠进行实验研究。Morris水迷宫定位航行实验结果显示,应激组子代与对照组相比,到达平台的潜伏期延长(P<0.05),而在空间探索实验中,应激组子代在原平台象限停留时间与对照组相比无显著差异。免疫组织化学结果显示,应激组雌性子代海马巢蛋白(nestin)和BrdU阳性细胞表达均较对照组显著增加(P<0.05),而雄性子代海马nestin和BrdU阳性细胞表达与对照组相比无显著性差异(P>0.05)。以上结果提示,产前束缚应激可引起雌性子代大鼠海马神经干细胞数量增加以及增殖能力增强,可能与机体对产前应激所致脑损伤的代偿性反应相关。
In order to observe the effect of prenatal restraint stress on spatial learning and memory, hippocampal neural stem cell proliferation and nestin expression, 12 Sprague-Dawley female rats weighing 240-260 g were randomly divided into two groups. The control group During pregnancy without any treatment, restraint stress group was given restraint stress at 14-20 d of pregnancy, 3 times / day, 45 min / times. One-month-old offspring rats were used for experimental study. Morris water maze localization navigation experiment results showed that compared with the control group, the offspring of the stress group had longer latency to reach the platform (P <0.05), while in the space exploration experiment, the stress group’s progenies stayed in the original platform quadrant with the control No significant difference between groups. The results of immunohistochemistry showed that the expression of nestin and BrdU positive cells in hippocampus of stress group were significantly higher than those in control group (P <0.05), while the expression of nestin and BrdU positive cells in male offspring hippocampus were similar to that of control group No significant difference (P> 0.05). These results suggest that prenatal restraint stress can cause female offspring rat hippocampal neural stem cells increased in number and increased proliferative capacity, may be related to the body and prenatal stress-induced brain injury compensatory response.