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目的探讨甲苯胺红不加热血清试验(TRUST)、酶联免疫吸附试验(TP-ELISA)和梅毒螺旋体明胶凝集试验(TPPA)3种试验方法在特殊人群梅毒检测中的应用价值。方法用TRUST和TP-ELISA2种方法同时检测398份血液标本,阳性者再用TPPA进行确证。结果 398份标本中TRUST法检测出阳性48例,阳性率为12.06%(48/398),经TPPA确证均为阳性,TP-ELISA法检测出阳性86例,阳性率为21.60%(86/398),经TPPA确证阳性82例,假阳性率4.65%(4/86),其中2种检测方法均为阳性的标本48例,TP-ELISA法阳性而TRUST法阴性的38例。结论 TP-ELISA法敏感性更高,操作简便,判断结果客观准确,可用于大批量标本初筛的首选方法;TRUST法适用于疗效观察及复发的辅助诊断;TP-ELISA法同时联合TURST、TPPA检测梅毒,对于避免梅毒的漏报、误报以及诊治有重要意义。
Objective To investigate the value of TRUST, TP-ELISA and TPPA in the detection of syphilis in special population. Methods Three hundred and eighty-eight blood samples were simultaneously detected by TRUST and TP-ELISA. Positive samples were confirmed by TPPA. Results The positive rate of TRUST in 398 specimens was 48 (39%). The positive rate was 12.06% (48/398). The positive rate was confirmed by TPPA. The positive rate was 86.6% (86/398) ). There were 82 positive cases confirmed by TPPA and the false positive rate was 4.65% (4/86). 48 of them were positive by two methods of detection, and 38 were positive by TP-ELISA and negative by TRUST. Conclusion The method of TP-ELISA is more sensitive and simple to operate, objective and accurate in judging the results. It can be used as the first choice method for screening large quantities of specimens. TRUST method is suitable for the diagnosis of relapse and secondary diagnosis. TP-ELISA combined with TURST and TPPA Detection of syphilis, to avoid syphilis omission, false positives and diagnosis and treatment are of great significance.