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目的研究黄连、吴茱萸水提物对二甲肼(DMH)诱导的大鼠结肠癌癌前病变的作用及其对结肠上皮增殖和凋亡的影响。方法采用DMH制备大鼠结肠癌模型;应用形态学的方法测定经过细微分离获得的完整肠腺内细胞的增殖和凋亡变化;应用甲基绿染色和光学显微镜计数中、降结肠异变肠腺病灶(ACF)数目。结果黄连、吴茱萸能够明显抑制模型组动物ACF的形成。模型组动物中、降结肠肠腺内细胞增殖显著增加,而黄连、吴茱萸能够明显抑制中、降结肠肠腺内细胞的增殖;模型组动物降结肠肠腺内细胞凋亡明显增加,而中结肠肠腺内细胞凋亡较正常相比无明显差异,而吴茱萸能够显著促进中、降结肠肠腺内细胞的凋亡,黄连无促进肠腺细胞凋亡的作用。结论黄连、吴茱萸能够显著抑制结肠癌癌前病变ACF的形成,提示其对结肠癌可能具有抑制和临床治疗作用,其中吴茱萸的作用部分通过抑制细胞增殖和促进细胞凋亡实现,而黄连对肠腺细胞的增殖具有抑制作用,但对肠腺细胞的凋亡无促进作用。
Objective To study the effect of aqueous extract of Coptis chinensis and Evodia rutaecarpa on precancerous lesions induced by dimethylhydrazine (DMH) in rats and its effect on the proliferation and apoptosis of colonic epithelium. Methods The rat model of colon cancer was established by DMH. Morphological methods were used to determine the proliferation and apoptosis of the intact glandular cells. The changes of apoptosis in the gut epithelium were observed by methyl green staining and optical microscopy. The number of lesions (ACF). Results Coptis, Evodia can significantly inhibit the formation of animal models of ACF. In model group, the proliferation of cells in the intestine of the descending colon increased significantly, and Coptis chinensis and Evodia could significantly inhibit the proliferation of the cells in the middle and descending colon glands. The apoptosis of the model group decreased significantly, Apoptosis in the gut gland compared with normal no significant difference, while Evodia can significantly promote the middle and lower intestinal gland cells in the apoptosis, and berberine did not promote intestinal gland cell apoptosis. Conclusions Coptis chinensis and Evodia could significantly inhibit the formation of ACF in precancerous lesions of colon cancer, suggesting that it may have an inhibitory and clinical therapeutic effect on colon cancer. The effect of Evodia rutaecarpa could be partly through inhibiting cell proliferation and promoting apoptosis. Proliferation inhibition, but no effect on the apoptosis of gut cells.