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为测定地肤子凝胶中 w(地肤子皂苷 Ic),采用 HPLC 法,色谱柱为 Agilent HC-C18(150 mm ×4.6 mm,5μm),流动相为甲醇-φ(冰醋酸)=1.3%水溶液(体积比85∶15),柱温20℃,流速1.0 mL/min,检测波长208 nm。测定中,ρ(地肤子皂苷 Ic)=98.4~246.0μg/mL,其峰面积与质量浓度呈良好的线性关系,样品平均回收率为97.7%,RSD =1.99%,测定3批样品中的平均 w(地肤子皂苷Ic)=0.564 mg/g。该方法快速简便,结果准确、可靠,重复性良好,可用于地肤子凝胶剂中 w(地肤子皂苷 Ic)的测定。“,”To establish a method for determination of Momordin Ic in Fructus Kochiae gel,the HPLC analysis was performed on a Agilent HC-C18 C18 column (150 mm×4.6 mm,5μm)with a mobile phase of methanol-1.3% acetic acid (85:15)at a flow rate of 1.0 mL/min,the detection wavelength was 208 nm,and the column temperature was 20 ℃.Good linearities of Momordin Ic was achieved in the con-centration ranges of 98.4~246.0 μg/mL.The average recovery of Momordin Ic was 97.7%,and the relative standard deviations was 1.99%.In three batches samples,the average content of Momordin Ic was 0.564 mg/g.The method was simple,accurate,reliable,reproducible,and can be used for quantita-tive determination of Momordin Ic in Fructus Kochiae gel.