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PAP技术即过氧化酶——抗过氧化酶法,对显示脑肠肽细胞及其定位具有较高敏感性。我们在免疫荧光组化方法基础上又建立此方法。 胃泌素是一种主要的脑肠肽,由胃泌素细胞合成、释放,在外周以胃窦分布最多,而中枢神经系统内是否有胃泌素存在,一直不能定论。不久前Loren等用免疫细胞方法证明大鼠脑中确有胃泌素细胞,我们实验室用RIA方法也测出狗中枢各脑区有胃泌素分布。本工作在此基础上用本实验室制备的高效特异免抗人胃泌素(G-17)血清,采用PAP方法观察猫胃窦部和大鼠中枢神经系统胃泌素细胞的分布。
The PAP technique, the peroxidase-anti-peroxidase method, is highly sensitive to displaying ghrelin cells and their localization. We constructed this method on the basis of immunofluorescence histochemistry. Gastrin is a major brain gut peptide, synthesized by gastrin cells, released in the outer most gastric antrum, and the central nervous system whether there is gastrin exist, has been inconclusive. Not long ago, Loren et al. Used immunocytochemistry to prove that there are indeed gastrine cells in the rat brain. In our laboratory, the RIA method was also used to determine the gastrin distribution in various brain regions of the dog’s center. In this work, we used the high-efficiency and special-free anti-human gastrin (G-17) serum prepared by our laboratory to observe the distribution of gastrin cells in the gastric antrum and the central nervous system of rats using PAP method.