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目的 研究TGF-β1对人晶状体上皮细胞(HLEC)上皮间质转分化(EMT)的影响.方法 根据处理不同,将细胞分为TGF-β1组、siRNA-TGF-β1组和空白对照组,培养24 h后观察细胞形态,Transwell法检测各组细胞迁移能力,实时荧光定量PCR检测各组细胞中TGF-β1、c-SMA、E-cadherin和vimentin基因表达,Western blotting法检测各组细胞中PI3K、Akt和p-Akt蛋白表达.结果 TGF-β1组迁移细胞数为(31.2±4.7)个,高于siRNA-TGF-β1组(14.6±5.3)个及空白对照组(3.9±1.4)个,且空白对照组高于siRNA-TGF-β1组;siRNA-TGF-β1组TGF-β1、α-SMA及Vimentin的mRNA相对表达量均低于TGF-β1组及空白对照组,且空白对照组低于TGF-β1组;siRNA-TGF-β1组E-cadherin的mRNA相对表达量均高于TGF-β1组及空白对照组,且空白对照组高于TGF-β1组;siRNA-TGF-β1组细胞中PI3K蛋白及p-Akt蛋白相对表达量均低于TGF-β1组和空白对照组,且空白对照组低于TGF-β1组,siRNA-TGF-β1组Akt蛋白相对表达量高于TGF-β1处理组和空白对照组,且空白对照组高于TGF-β1组.结论 TGF-β1可诱导HLEC发生EMT,且可促进HLEC迁移,其机制可能与细胞中PI3K/Akt信号通路活化有关.“,”Objective To investigate the effect of transforming growth factor (TGF)-β1 on the epithelial-mesenchymal transition (EMT) in human lens epithelial cells (HLECs).Methods HLECs were cultured separately,according their treatment group.For this purpose,cells were divided into the TGF-β1 treatment group,siRNA-TGF-β1 group,and the control group.After culturing cells for 24 h in their respective treatment groups,cell morphologies were observed.Cell migration in each treatment group was detected using the transwell migration assay.The expression levels of TGF-β1,α-SMA,E-cadherin,and vimentin genes were detected using real-time polymerase chain reaction.The expression levels of PI3K,Akt,and p-Akt proteins were detected using Western blotting.Results In the TGF-β1 treatment group,31.2 ± 4.7 HLECs were found to have transformed.This number was significantly higher than that in the siRNA-TGF-β1 (14.6 ± 5.3) and control groups (3.9 ± 1.4).Specifically,the number of cells transformed in the siRNA-TGF-β1 group was higher than that in the control group.Expression levels of TGF-β1 mRNA,α-SMA mRNA,and vimentin mRNA were the highest in the TGF-β1 treatment group,followed by lower levels in the siRNA-TGF-β1 group,and the lowest levels in the control group.In contrast,the expression levels of E-cadherin mRNA were highest in the siRNA-TGF-β1 group,followed by lower levels in the control group and the lowest levels in the TGF-β1 treatment group.These differences were statistically significant (P ＜ 0.05).The expression levels of PI3K and p-Akt proteins were the highest in the cells of the TGF-β1 treatment group,followed by lower levels in the control group.These levels were lowest in the siRNA-TGF-β1 group.The relative expression levels of Akt proteins were the highest in the siRNA-TGF-β1 group,followed by lower levels in the control group and the lowest levels in the TGF-β1 treatment group;these differences were statistically significant (P ＜ 0.05).Conclusion TGF-β1 could induce HLECs towards EMT,and thus promote HLEC migration.This mechanism could be related to the activation of PI3K/Akt signaling pathway.