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目的:研究鼻咽癌患者联合放化疗前后CD4+/CD25+与CD28+/TCR+T淋巴细胞亚群的变化。方法:招募43例接受联合放化疗的鼻咽癌患者,在联合放化疗前、联合放化疗3周及6周后分别抽取外周静脉血,运用葡聚糖-泛影葡胺密度梯度离心法分离并收集淋巴细胞,流式细胞仪检测CD4+/CD25+与CD28+/TCR+T淋巴细胞亚群的比例。结果:联合放化疗3周及6周后CD4+/CD25+淋巴亚群无明显变化(P>0.05),CD28+/TCR+淋巴亚群明显升高(P<0.05)。结论:联合放化疗可部分恢复鼻咽癌患者受抑制的免疫功能。
Objective: To study the changes of CD4 + / CD25 + and CD28 + / TCR + T lymphocyte subsets in patients with nasopharyngeal carcinoma before radiotherapy and chemotherapy. Methods: Thirty-three patients with nasopharyngeal carcinoma who underwent radiotherapy and chemotherapy were enrolled. Peripheral venous blood was collected before radiotherapy combined with chemoradiotherapy 3 weeks and 6 weeks after radiotherapy and chemotherapy, respectively, and dextran-diacetate meglumine gradient centrifugation Lymphocytes were collected and the ratio of CD4 + / CD25 + to CD28 + / TCR + T lymphocyte subsets was detected by flow cytometry. Results: There was no significant change in CD4 + / CD25 + lymphocyte subsets between 3 and 6 weeks after radiotherapy and chemotherapy (P> 0.05). The lymphocyte subsets of CD28 + / TCR + cells were significantly increased (P <0.05). Conclusion: Radiotherapy combined with radiotherapy can partly restore the suppressed immune function in patients with nasopharyngeal carcinoma.