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目的:探讨高危型人乳头状瘤病毒(HPV)16/18型感染及整合状态对广东地区喉癌发病的意义。方法:应用实时定量聚合酶链反应(PCR)检测2010年1月至2012年6月广东省人民医院耳鼻咽喉头颈外科喉癌石蜡包埋标本中HPV16/18 E2、E6的拷贝数,分析HPV16/18病毒感染情况和整合状态,并对其进行随访。结果:82例喉癌标本中,2例HPV16阳性(2.4%),整合状态为游离型或混合型,未发现HPV完全整合入宿主DNA者。HPV阳性2例患者存活至今无复发。结论:高危型HPV16/18在喉癌中的感染率较低,HPV16/18可能不是广东地区喉癌的主要发病因素,对喉癌的预后影响可能有限。
Objective: To investigate the significance of high risk HPV 16/18 infection and its integrated status in the pathogenesis of laryngeal cancer in Guangdong Province. METHODS: The copy numbers of HPV16 / 18 E2 and E6 in paraffin-embedded specimens of laryngeal carcinoma of the otolaryngology and head and neck surgery department of Guangdong Provincial People’s Hospital from January 2010 to June 2012 were detected by real-time quantitative polymerase chain reaction (PCR) 18 virus infection and the status of integration, and its follow-up. Results: Of the 82 laryngeal cancer samples, 2 were positive for HPV16 (2.4%) and the integrated status was either free form or mixed form. No HPV was found to be completely integrated into the host DNA. Two patients with HPV positive survived to no recurrence. CONCLUSION: High-risk HPV16 / 18 has a low infection rate in laryngeal cancer. HPV16 / 18 may not be the major causative factor for laryngeal cancer in Guangdong Province. The prognosis of laryngeal cancer may be limited.