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目的观察基质细胞衍生因子-1(SDF-1)对糖尿病患者外周血内皮祖细胞(EPCs)功能的影响。方法选择糖尿病患者(DM组)20例和健康体检者(NC)10名,获取外周血单个核细胞,培养4d后鉴定EPCs。将两组随机分为NC1、NC2、DM1和DM2亚组。NC1、DM1亚组采用SDF-1进行干预,第7天检测EPCs迁移能力和血管形成能力。结果 (1)DM2亚组EPCs迁移和血管形成能力均低于NC2亚组[(15.500±2.224)vs(21.200±1.304)个,(113.870±15.198)vs(181.800±9.202)μm,P<0.001];与NC2亚组比较,NC1亚组EPCs迁移能力和血管形成能力均增强[(24.600±1.517)vs(21.200±1.304)个,(197.980±10.855)vs(181.800±9.202)μm,P<0.05];与DM2亚组比较,DM1亚组EPCs迁移能力和血管形成能力增强[(19.300±1.337)vs(15.500±2.224)个,(140.520±10.622)vs(113.870±15.198)μm,P<0.001];(2)加入SDF-1干预后,DM组EPCs迁移能力和血管形成能力增强的幅度均高于NC组,比较差异有统计学意义(P=0.036,0.006);(3)SDF-1与EPCs迁移能力及血管形成能力呈正相关(r=0.488、0.428,P=0.006、0.018)。结论 SDF-1可增强外周血EPCs迁移能力和血管形成能力,对于糖尿病患者效果更为明显。
Objective To observe the effect of stromal cell-derived factor-1 (SDF-1) on the function of endothelial progenitor cells (EPCs) in peripheral blood of diabetic patients. Methods Twenty diabetic patients (DM group) and 10 healthy control subjects (NC) were selected to obtain peripheral blood mononuclear cells (EPCs). EPCs were identified after 4 days of culture. The two groups were randomly divided into NC1, NC2, DM1 and DM2 subgroups. The NC1 and DM1 subgroups were intervened by SDF-1, and the EPCs migration and angiogenesis were detected on the 7th day. Results (1) EPCs migration and angiogenesis in DM2 subgroup were lower than those in NC2 subgroup [(15.500 ± 2.224 vs 21.200 ± 1.304, (113.870 ± 15.198) vs (181.800 ± 9.202) μm, P <0.001) ; Compared with NC2 subgroup, the migration and angiogenesis of EPCs in NC1 subgroup were enhanced [(24.600 ± 1.517) vs (21.200 ± 1.304) vs (197.980 ± 10.855 vs 181.800 ± 9.202 μm, P <0.05] (19.300 ± 1.337 vs 15.500 ± 2.224, (140.520 ± 10.622) vs (113.870 ± 15.198) μm, respectively, P <0.001]. Compared with the DM2 subgroup, the migration and angiogenesis of EPCs in DM1 subgroup were enhanced (2) After the intervention of SDF-1, the migration ability and angiogenic capacity of EPCs in DM group were higher than those in NC group (P = 0.036,0.006); (3) SDF-1 and EPCs Migration capacity and angiogenesis ability were positively correlated (r = 0.488,0.428, P = 0.006,0.018). Conclusion SDF-1 can enhance EPCs migration and angiogenesis in peripheral blood, which is more effective for diabetic patients.