近来报道两性霉素B在延长田鼠大脑内感染搔痒症潜伏期中起着有利影响。本文报道了测定田鼠脑组织中低含量两性霉素B的简单而又灵敏的分光光度法。两性霉素B脂质体在40℃贮存最多为3天,在给动物服用之前。用磷酸盐缓冲盐水适当稀释。组织中的两性霉素B用Mayhew等提出的方法进行提取并稍作改进。动物服氯仿杀死,取出脑和脾,称量,在冷冻下加入甲醇3ml匀浆器中进行匀浆。匀浆在50℃水浴中加热20min,然后在3 500×g下离心30min,上清液用甲醇调节最后的体积到5ml,再离心15min,在波长330和430nm下扫描测 It has recently been reported that amphotericin B plays a beneficial role in prolonging the incubation period for pruritus infection in the voles of the voles. This article reports a simple and sensitive spectrophotometric method for the determination of low levels of amphotericin B in the brain tissue of voles. Amphotericin B liposomes are stored at 40 ° C for up to 3 days prior to administration to animals. Appropriate dilution with phosphate buffered saline. Amphotericin B in the tissue was extracted and slightly modified using the method proposed by Mayhew et al. The animals were killed with chloroform, the brain and spleen were removed, weighed, and homogenized by adding 3 ml of methanol in a homogenizer under freezing. The homogenate was heated in a water bath at 50 ° C for 20 min and then centrifuged at 3 500 × g for 30 min. The supernatant was adjusted to a final volume of 5 ml with methanol, centrifuged again for 15 min, and scanned at wavelengths of 330 and 430 nm