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膀胱癌是发生在膀胱黏膜组织上的一种恶性肿瘤,是泌尿系统中最常见的恶性肿瘤,早期(非肌层浸润型膀胱癌)阶段的诊断和治疗是降低膀胱癌死亡率的最有效方式.肿瘤的发生过程与糖链表达的改变有着密切的关系,而定量分析膀胱癌发生过程中糖链的表达变化尚未有研究.本研究以2株人膀胱正常上皮细胞系(HCV29、HUCV1),1株非肌层浸润性膀胱癌细胞系(KK47),和3株浸润性膀胱癌细胞系(YTS1、J82、T24)为研究材料,应用本室建立的利用乙酰肼修饰糖链唾液酸,以及[12C6]-和[13C6]-苯胺同位素修饰糖链还原性末端技术,然后利用基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS),进行膀胱上皮细胞不同病理状态的糖组相对定量分析.从6株细胞中共鉴定出52种N-连接糖链结构,并定量分析了不同类型的糖链在不同细胞中的分布差异,发现唾液酸化、岩藻糖化的N-连接糖链在膀胱癌肿瘤细胞恶化过程中呈现显著升高的趋势,同时平分型糖链和高甘露糖型N-连接糖链也呈表达升高趋势,说明这些糖链结构的表达变化与膀胱癌发生关系密切,从而有助于进一步阐明膀胱癌发生过程中糖链相关的分子机理.
Bladder cancer is a malignant tumor that occurs in bladder mucosa tissue and is the most common malignancy in the urinary system. The most effective way to reduce the mortality of bladder cancer is to diagnose and treat early stage (non-muscle invasive bladder cancer) stage There is a close relationship between the occurrence of cancer and the changes of the expression of sugar chain, but there are still no studies on the quantitative analysis of the changes of the expression of sugar chain during the development of bladder cancer.In this study, two human bladder normal epithelial cell lines (HCV29, HUCV1) One non-muscle invasive bladder cancer cell line (KK47) and three invasive bladder cancer cell lines (YTS1, J82 and T24) were used as materials for the study. The acetylcholine-modified sugar chain sialic acid The [12C6] - and [13C6] -aniline isotope modified sugar chain reducing end technology, and then using matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS), the relative quantitative analysis of different pathological state of bladder epithelial cells .A total of 52 N-linked sugar chains were identified from 6 cell lines, and the distribution of different types of sugar chains in different cells was quantitatively analyzed. It was found that sialylation and fucosylation of N-linked sugar chains in bladder cancer swollen Cell deterioration showed a trend of significant increase in the same time, the type of homozygous and high-mannose N-linked sugar chain also showed an upward trend in expression, indicating that these changes in the expression of sugar chain and bladder cancer are closely related to Help to further clarify the molecular mechanism of sugar chain in the process of bladder cancer.