论文部分内容阅读
目的 研究黄芪注射液 (AMI)对贫血小鼠巨核系造血的影响及其作用机制。方法 制备贫血小鼠模型 ,随机分为给药组和对照组 ,分别腹腔注射 AMI〔2 0 m g/ (ml· 2 0 g)〕或等量生理盐水 q.d× 6 ,于给药后第8、11、14天常规方法检测外周血血小板数、骨髓有核细胞数 ,同时用体外巨核系祖细胞 (CFU- Meg)培养方法检测CFU- Meg,采集血清作巨核细胞系集落刺激活性 (Meg- CSA)测定。结果 贫血小鼠给药组和对照组比较 ,血清Meg- CSA明显升高 ,给药后第 11天所测各项指标均恢复正常 ,恢复时间明显缩短。结论 AMI能够升高血清Meg- CSA,加速骨髓抑制后巨核系造血功能的恢复
Objective To study the effect and mechanism of Huangqi injection (AMI) on hematopoiesis of megakaryocyte in anemic mice. METHODS An anemic mouse model was prepared and randomly divided into administration group and control group. AMI [20 mg/(ml·20 g)] or equal volume of normal saline qd×6 was intraperitoneally injected respectively. Conventional methods were used to detect the number of peripheral blood platelets and the number of bone marrow nucleated cells on the 11th and 14th days. At the same time, CFU-Meg was detected in vitro using a megakaryocyte progenitor cell (CFU-Meg) culture method, and serum was collected for megakaryocyte cell colony stimulating activity (Meg-CSA). ) Determination. Results Compared with the control group, the serum Meg-CSA was significantly increased in the anemia mouse administration group, and all the indexes measured on the 11th day after administration were normal, and the recovery time was significantly shortened. Conclusion AMI can increase serum Meg-CSA and accelerate the recovery of megakaryocyte hematopoietic function after myelosuppression