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目的探讨长链非编码RNA尿路上皮癌相关1(UCA1)在肝癌肝内转移患者肿瘤组织和血浆中的表达及临床意义。方法收集2015年1月至2016年1月第二军医大学长海医院确诊为肝癌肝内转移并手术切除的20例患者的肿瘤组织和术前、术后血浆样本,20例肝癌患者的肿瘤组织和血浆样本,以及20例健康受试者的血浆样本。采用q PCR法测定肿瘤组织及血浆样本中UCA1 mRNA的表达,并分析UCA1 mRNA表达与Child-Pugh分级、原发灶肿瘤大小、年龄、性别、饮酒史等临床病理特征的关系。结果肝癌肝内转移患者肿瘤组织与血浆中UCA1 mRNA表达均高于肝癌患者(P<0.01),术后血浆中UCA1 mRNA表达低于术前(P<0.05)。肝癌肝内转移患者肿瘤组织UCA1 mRNA的表达与原发灶肿瘤大小(P=0.025)和Child-Pugh分级(P=0.006)有关。结论血浆UCA1可能是肝癌转移诊断和病情监测的标志物,UCA1的表达水平与肝癌发展密切相关。
Objective To investigate the expression and clinical significance of long-chain non-coding RNA in urothelial carcinoma-associated 1 (UCA1) in patients with intrahepatic metastasis of liver cancer. Methods The tumor tissues, preoperative and postoperative plasma samples of 20 patients diagnosed as intrahepatic metastasis and surgical resection of liver cancer from January 2015 to January 2016 in Changhai Hospital of Second Military Medical University were collected. Tumor tissues of 20 patients with HCC Plasma samples, and plasma samples from 20 healthy subjects. QPCR method was used to detect the expression of UCA1 mRNA in tumor tissue and plasma samples. The relationship between UCA1 mRNA expression and clinicopathological features such as Child-Pugh grading, tumor size, age, gender and drinking history were analyzed. Results The expression of UCA1 mRNA in tumor tissues and plasma of patients with intrahepatic metastasis of hepatocellular carcinoma was higher than that of hepatocellular carcinoma patients (P <0.01). The expression of UCA1 mRNA in plasma after operation was lower than that before operation (P <0.05). The expression of UCA1 mRNA in patients with intrahepatic metastasis of HCC was correlated with the size of primary tumor (P = 0.025) and Child-Pugh classification (P = 0.006). Conclusions Plasma UCA1 may be a marker of liver cancer metastasis and disease monitoring. The expression level of UCA1 is closely related to the development of liver cancer.