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以CH_3I为甲基化试剂、NaOH为填料的填充柱对麦芽三糖进行固相甲基化,利用液相色谱-质谱(LC-MS)检测甲基化产物并优化甲基化条件。最优条件为4.6 mm×150 mm的不锈钢填充柱,流速40μL/min和柱温25℃,在此条件下分别对麦芽糖、蔗糖、蔗果四糖及人参寡糖提取物进行固相甲基化,并以蔗果四糖为例进行方法学考察。蔗果四糖的线性范围为10.8~5400 ng/mL,r~2=0.9974,平均加标回收率为122.5%。
Maltotriose was subjected to solid-phase methylation with CH_3I as methylating reagent and NaOH as packed column. The methylation products were detected by LC-MS and the methylation conditions were optimized. The optimum conditions were 4.6 mm × 150 mm stainless steel packed column, the flow rate was 40 μL / min and the column temperature was 25 ℃. Under these conditions, maltose, sucrose, cane sugar and ginseng oligosaccharide extracts were subjected to solid phase methylation , And using cane fructose as an example for methodological study. The linear range of cane sugar was 10.8 ~ 5400 ng / mL, r ~ 2 = 0.9974, and the average recovery was 122.5%.