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以长白落叶松成熟胚作为外植体进行消毒和不定芽诱导。研究结果表明:将去皮种子用流水冲洗3d,用70%酒精消毒1min,转入1%的次氯酸钠消毒50min后,再将剥出的胚放入0.5%的次氯酸钠中浸泡2s为最佳消毒方法;在光照条件下(约1600lx),BM培养基中填加1.0mg/LBA,愈伤组织与不定芽的诱导效果最好,愈伤组织诱导率达到100%,不定芽分化率为73.8%;在BM培养基中加入1%活性碳对不定芽有伸长作用。
The mature embryos of Larix olgensis were used as explants for disinfection and adventitious bud induction. The results showed that: the peeled seeds were washed with water for 3 days, disinfected with 70% alcohol 1min, 1% sodium hypochlorite transferred to disinfection 50min, and then stripped of embryos into 0.5% sodium hypochlorite soaked 2s for the best method of disinfection Under the light conditions (about 1600lx), BM medium supplemented with 1.0mg / LBA, callus induction and adventitious buds the best, callus induction rate of 100%, adventitious bud differentiation rate was 73.8%; Addition of 1% activated carbon to BM medium has the effect of adventitious bud elongation.