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目的:研究BCL2L2、CCNA1、CD44、CTSD和MAP2K7基因表达与乳腺癌复发转移特性的关系。方法:81例乳腺癌患者术后5年随访期内出现复发者为研究组(41例)和未复发仍存活者为对照组(40例)。提取所有病例石蜡包埋组织的RNA,质量鉴定后,应用实时定量RT-PCR芯片技术平行检测BCL2L2、CCNA1、CD44、CTSD和MAP2K7基因的表达,分析各基因表达差异与乳腺癌复发转移之间的关系。结果:CCNA1、CD44和MAP2K7基因在复发病例中均高表达(57.5%、66.7%和58.5%),而BCL2L2和CTSD基因在复发组中低表达(56.1%和53.7%),BCL2L2、CCNA1、CD44、CTSD和MAP2K7基因表达水平在复发与未复发组间差异均有统计学意义,P均<0.05。BCL2L2和CTSD基因是乳腺癌复发转移的危险因素。CCNA1、CD44和MAP2K7基因对乳腺癌复发转移是保护因素。结论:实时定量RT-PCR芯片技术能快速、准确地检测肿瘤相关特性基因AR、CC-ND1和ESR2的表达。乳腺癌术后BCL2L2、CCNA1、CD44、CTSD和MAP2K7基因的差异表达分析可做为预防和控制该病复发的新线索。
Objective: To study the relationship between BCL2L2, CCNA1, CD44, CTSD and MAP2K7 gene expression and the recurrence and metastasis of breast cancer. Methods: Eighty-one breast cancer patients were recruited as study group (41 cases) and non-recurrent survivors as control group (40 cases) after 5-year follow-up. The RNA of paraffin-embedded tissues was extracted from all the cases. After the quality was identified, the expression of BCL2L2, CCNA1, CD44, CTSD and MAP2K7 genes were detected by real-time quantitative RT-PCR and the difference between the gene expression and the recurrence and metastasis of breast cancer relationship. Results: The expression of CCNA1, CD44 and MAP2K7 were all significantly higher in relapsed cases (57.5%, 66.7% and 58.5%), while BCL2L2 and CTSD genes were lower (56.1% and 53.7%) in BCL2L2, CCNA1 and CD44 There was significant difference in the expression of CTSD and MAP2K7 between recurrence and non-recurrence (P <0.05). BCL2L2 and CTSD genes are risk factors for recurrence and metastasis of breast cancer. The CCNA1, CD44 and MAP2K7 genes are protective factors of breast cancer recurrence and metastasis. Conclusion: Real-time quantitative RT-PCR chip technology can rapidly and accurately detect the expression of tumor related gene AR, CC-ND1 and ESR2. The differential expression analysis of BCL2L2, CCNA1, CD44, CTSD and MAP2K7 genes after breast cancer can be used as a new clue to prevent and control the recurrence of the disease.