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目的对福氏2a志贺菌肠毒素ShET1(Shigellaenterotoxin1)和肠毒素ShET2(Shigellaenterotoxin2)进行基因分型,提高菌痢爆发流行时同源克隆的鉴定分析水平。方法对93株分离自不同地区,不同时间的福氏2a志贺菌用PCR法检测志贺菌肠毒素ShET1/ShET2基因,进行基因分型和同源克隆鉴定。结果93株福氏2a志贺菌按志贺菌肠毒素ShET1/ShET2基因可分为4种基因型,即12株ShET1(-)/ShET2(+),14株ShET1(+)/ShET2(-),59株ShET1(+)/ShET2(+),8株ShET1(-)/ShET2(-)。93株福氏2a志贺菌ShET1检出率为89.24%(83/93),ShET2为65.59%(61/93)。二者至少有一种基因被检出的检出率为91.39%(85/93)。结论福氏2a志贺菌的快速诊断可应用ShET1、ShET2双基因PCR检测,具有较高的敏感性与特异性。在应用多生物学标志进行福氏2a志贺菌同源克隆鉴定系统研究时,肠毒素ShET1/ShET2基因PCR分析是不可或缺的分析指标。
Objective To genotype Shigellaenterotoxin1 and Shigellaenterotoxin2 of Shigella flexneri 2a in order to improve the identification and analysis of homologous clones in the epidemic of bacillary dysentery. Methods Ninety-three Shigella flexneri 2a Shigella flexneri isolates from different regions and different times were used to detect Shigella enterotoxin ShET1 / ShET2 gene by PCR and genotyped and identified by homologous cloning. Results Ninety - three Shigella spp. Shigella were divided into four genotypes according to ShET1 / ShET2 gene, that is, 12 ShET1 (-) / ShET2 (+) and 14 ShET1 (+) / ShET2 ), 59 ShET1 (+) / ShET2 (+) and 8 ShET1 (-) / ShET2 (-). 93 strains of Shigella flexneri ShET1 detection rate was 89.24% (83/93), ShET2 was 65.59% (61/93). The detection rate of at least one of the two genes was 91.39% (85/93). Conclusion The rapid diagnosis of Shigella flexneri 2a can be detected by ShET1 and ShET2 double gene PCR, which has high sensitivity and specificity. The PCR analysis of ShET1 / ShET2 genes of enterotoxigenic toxins is an indispensable index in the application of multiple biological markers for the identification of Shigella flexneri 2a.