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目的 研究人参皂甙对内毒素脂多糖 (lipopolisaccharide ,LPS)致血管内皮细胞纤溶酶原激活物抑制剂 1(plasminogenactivatorinhibitortype 1,PAI 1)表达和核因子 κB (NF κB)激活的影响 ,探讨人参皂甙对心血管疾病的保健和防治机制。方法 用酶消化法培养人脐静脉内皮细胞(humanumbilicalveinendothelialcells ,HUVEC) ;ELISA方法检测HUVEC条件培养液PAI 1蛋白量 ;Northernblot检测HUVECPAI 1的mRNA表达 ;免疫荧光检测HUVECNF κB的分布 ;电泳迁移变动检测法检测HUVEC核提取物NF κBDNA结合活性。结果 LPS能使HUVECPAI 1蛋白及mRNA表达显著增强 ,人参皂甙则使LPS的这种作用明显减弱。免疫荧光显示 ,LPS促使HUVECNF κB向细胞核内转移 ,人参皂甙则能阻止LPS诱导的NF κB核内转移 ;电泳迁移变动检测法研究发现 ,人参皂甙能完全阻止LPS致内皮细胞核提取物NF κBDNA结合活性。结论 人参皂甙对心血管疾病的防治机制之一是通过拮抗LPS致HUVECPAI 1的表达。而且 ,这种拮抗作用可能通过NF κB途径来转导。
Objective To investigate the effects of ginsenosides on the expression of plasminogen activator inhibitor of typhimurium 1 (PAI 1) and the activation of nuclear factor κB (NF κB) induced by lipopolysaccharide (LPS) in rats. To investigate the effects of ginsenosides Cardiovascular health care and prevention mechanisms. Methods Human umbilical vein endothelial cells (HUVECs) were cultured by enzymatic digestion method. PAI 1 protein in HUVEC conditioned medium was detected by ELISA. The mRNA expression of HUVECPAI 1 was detected by Northern blot. The distribution of NFκB in HUVECs was detected by immunofluorescence. HUVEC nuclear extract NF κB DNA binding activity was detected. Results LPS enhanced the expression of HUVECPAI 1 protein and mRNA, while ginsenosides significantly reduced the effect of LPS. Immunofluorescence showed that LPS promoted the transfer of NF-κB in HUVEC into the nucleus and Ginsenoside blocked LPS-induced NF-κB nuclear translocation. The results of electrophoretic mobility shift assay showed that Ginsenoside could completely block NF-κB DNA binding activity of LPS-induced endothelial cells . Conclusion Ginsenoside is one of the mechanisms of prevention and treatment of cardiovascular diseases by antagonizing the expression of HUVECPAI 1 induced by LPS. Moreover, this antagonism may be transduced through the NFKB pathway.