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目的:探讨黄精多糖(PSP)促进小鼠骨髓基质细胞(BMSCs)生长及干预长春新碱(VCR)对小鼠BMSCs增殖的抑制。方法:①PSP 0.5、1.0、2.0、4.0g/L,以及VCR 2.5、5.0、10、15mg/L分别与骨髓单个核细胞(BMMCs)共同培养14d,应用MTT法测定BMSCs增殖。②PSP 0.5、1.0、2.0、4.0g/L与BMMCs预培养2h后再分别加入终浓度为5.0mg/L的VCR继续培养14d,应用MTT法测定BMSCs增殖。结果:BMSCs培养14d后,PSP 1.0、2.0、4.0g/L组的OD值(3.80、4.48、4.05)均高于正常对照组(3.70),其中PSP 2.0g/L组OD值与正常对照组比较差异均有统计学意义(P<0.05)。VCR 5.0、10.0、15.0mg/L 3组OD值(2.36、1.83、1.67)均显著低于正常对照组(3.70)(P<0.05)。BMSCs培养第14d PSP 2.0g/L+VCR 5.0mg/L组OD值(3.51)与VCR 5.0mg/L组OD值(2.62)比较差异有统计学意义(P<0.05)。结论:VCR明显抑制BMSCs生长;PSP可促进BMSCs生长,且干预VCR对BMSCs增殖的抑制。
Objective: To investigate the effects of Polygonatum Polysaccharide (PSP) on the growth of mouse bone marrow stromal cells (BMSCs) and the inhibition of vincristine (VCR) on the proliferation of mouse BMSCs. Methods: ①PSP 0.5,1.0,2.0,4.0 g / L and VCR 2.5,5.0,10,15 mg / L were cultured with bone marrow mononuclear cells (BMMCs) for 14 days. The proliferation of BMSCs was measured by MTT assay. ②PPS 0.5,1.0,2.0,4.0 g / L and BMMCs were pre-cultured for 2h and then were added to a final concentration of 5.0mg / L VCR continue to culture for 14 days, MTT assay BMSCs proliferation. Results: The OD values of PSP 1.0, 2.0 and 4.0 g / L group (3.80, 4.48, 4.05) were higher than those of normal control group (3.70) The differences were statistically significant (P <0.05). The OD values of VCR 5.0,10.0,15.0mg / L 3 group (2.36,1.83,1.67) were significantly lower than that of the normal control group (3.70) (P <0.05). There was significant difference between OD value (3.51) of PSP 2.0g / L + VCR 5.0mg / L group and OD value of VCR 5.0mg / L group (2.62) on the 14th day of BMSCs culture (P <0.05). CONCLUSION: VCR can significantly inhibit the growth of BMSCs. PSP can promote the growth of BMSCs and inhibit the proliferation of BMSCs by VCR.