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本研究评估供体的心脏经辅酶Q_(10)预处理能否促进心肌缺血后的恢复。辅酶Q_(10)或辅酶Q_(10)的溶剂(作为对照)在心脏离体之前一小时注射到犬的静脉内。方法是将每个离体的心脏浸泡在4℃ Euro-Collins溶液中6小时或24小时。然后用支持犬的动脉血再灌注到主动脉内,在37℃条件下维持3小时。在保存期间即在心脏保存6小时和24小时之后,对照组ATP水平显著减少,每克干重从21.0μmol分别降到15.1μmol和11.9μmol。用辅酶Q_(10)预处理使心脏保存24小时后的ATP水平维持在18.9μmol。心脏保存6小时和24小时进行再灌注后,辅酶Q_(10)预处理组的ATP可恢复到原来的水平。但对照组仍然处于低水平。对照组心脏保存
This study assessed whether donor heart preconditioning with coenzyme Q_ (10) could promote myocardial ischemia recovery. The solvent of coenzyme Q_ (10) or coenzyme Q_ (10) (as a control) was injected into the canine vein one hour before ex vivo. The method is to soak each of the isolated hearts in a Euro-Collins solution at 4 ° C for 6 hours or 24 hours. Dogs were then re-infused into the aorta with artery blood and maintained for 3 hours at 37 ° C. After 6 h and 24 h of preservation in the heart, the ATP level of the control group was significantly reduced from 21.0 μmol to 15.1 μmol and 11.9 μmol per gram of dry weight, respectively. Pretreatment with coenzyme Q_ (10) maintained the ATP level at 18.9 μmol 24 hours after cardiac preservation. After reperfusion at 6 hours and 24 hours after cardiac preservation, ATP in coenzyme Q_ (10) pretreatment group returned to its original level. However, the control group remained at a low level. Control group heart preservation