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目的研究Mtb耐INH临床分离株katG基因、inhA基因、ahpC基因突变对其体外最小抑菌浓度(MIC)的影响。方法对临床分离的耐INH的160株Mtb及对INH敏感的40株Mtb进行耐药基因芯片检测;并对所有标本进行INH MIC测定,比较不同变异株的MIC结果,其数据采用t检验进行比较,以P<0.05为差异有统计学意义。结果Mtb耐INH株katG315基因变异占68.8%(110/160),katG315基因变异株的MIC为(20.20±19.46μg/ml),高于inhA变异株(0.73±0.82μg/ml)(t′=10.9171,t′α=1.9807,P<0.05);低于katG315+inhA变异株(69.33±32.45μg/ml),(t′=7.164,t′α=2.0627,P<0.05)。结论 Mtb耐INH基因有不同的突变模式,各模式存在不同的耐药程度,此可以给临床用药进一步提供参考。
Objective To investigate the effect of mutations in katG gene, inhA gene and ahpC gene on minimal inhibitory concentration (MIC) in Mtb resistant INH strains. Methods 160 isolates of INH resistant to MtH and 40 isolates of INH sensitive to Mtb were detected by drug resistance microarray. INH MIC was measured on all the samples, and the MIC of different strains was compared. The data were compared by t test , P <0.05 for the difference was statistically significant. Results The katG315 gene mutation in Mtb resistant INH strains accounted for 68.8% (110/160), the katG315 gene variant strains (20.20 ± 19.46μg / ml) were higher than inhA mutants (0.73 ± 0.82μg / ml) (t ’ (T ’= 7.164, t’α = 2.0627, P <0.05), which was lower than that of katG315 + inhA mutant (69.33 ± 32.45μg / ml). Conclusion Mtb resistant INH gene has different mutation patterns and different patterns of resistance, which can provide reference for further clinical use.