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目的探讨白藜芦醇(Res)对脂多糖(LPS)诱导小鼠腹腔巨噬细胞核因子-κB(NF-κB)活化及炎性细胞因子[肿瘤坏死因子(TNF-α)、白介素-1β(IL-1β)、白介素-6(IL-6)]基因表达的调节。方法分别用1mg/LLPS或25mmol/LRes+1mg/LLPS处理体外培养的小鼠巨噬细胞,采用电泳迁移率改变分析法(EMSA)检测细胞中NF-κB活性,逆转录-聚合酶链反应(RT-PCR)和酶联免疫吸附法(ELISA)检测细胞中TNF-α、IL-1β、IL-6mRNA和蛋白的表达。结果LPS组NF-κB活性和TNF-α、IL-1β、IL-6含量在刺激后6～12h明显高于正常对照组(P<0.001),而Res+LPS组NF-κB活性和TNF-α、IL-1β、IL-6含量均显著低于LPS组(P<0.005)。结论LPS可诱导巨噬细胞NF-κB活化,导致TNF-α、IL-1β、IL-6基因表达增强,而Res能抑制NF-κB活化而调节TNF-α、IL-1β、IL-6基因的表达。
Objective To investigate the activation of nuclear factor-κB (NF-κB) in mouse peritoneal macrophages induced by resveratrol (Res) and the inflammatory cytokines (tumor necrosis factor-α, interleukin-1β) induced by lipopolysaccharide (LPS). Regulation of IL-1β), Interleukin-6 (IL-6) Gene Expression. Methods Mouse macrophages cultured in vitro were treated with 1 mg/LLPS or 25 mmol/LRes + 1 mg/LLPS. The activity of NF-κB in cells was detected by electrophoretic mobility shift assay (EMSA) and RT-PCR. RT-PCR and enzyme-linked immunosorbent assay (ELISA) were used to detect the expression of TNF-α, IL-1β, IL-6 mRNA and protein in the cells. Results The activity of NF-κB and the contents of TNF-α, IL-1β and IL-6 in LPS group were significantly higher than those in normal control group at 6-12 h (P<0.001), while the activity of NF-κB and TNF- The content of α, IL-1β and IL-6 was significantly lower than that of LPS group (P<0.005). Conclusion LPS can induce NF-κB activation in macrophages, resulting in enhanced expression of TNF-α, IL-1β and IL-6 genes. Res can inhibit NF-κB activation and regulate TNF-α, IL-1β and IL-6 genes. expression.