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目的:比较桔梗和牛膝不同配伍对正常和血瘀小鼠体内芍药苷组织分布的影响,研究桔梗和牛膝的引经作用。方法:正常和血瘀小鼠分别灌胃血府逐瘀汤全方、单缺桔梗方、单缺牛膝方、缺桔梗和牛膝方,30 min后处死,收集全血,取出脑、心、肝、脾、肺、肾。采用HPLC测定芍药苷含量,流动相乙腈-水(16∶84),检测波长230 nm,栀子苷为内标物。结果:正常组小鼠分别灌胃血府逐瘀汤全方、单缺桔梗方、单缺牛膝方、缺桔梗和牛膝方后,脑、心、肝、脾中均未检测到芍药苷,肺中芍药苷质量分数分别为0.010 2,0.005 8,0.091 0,0.037 3μg·g-1,肾中芍药苷质量分数分别为0.122 2,0.052 0,0.144 0,0.065 0μg·g-1。血瘀小鼠灌胃血府逐瘀汤全方和单缺桔梗方后,脑中均未检测到芍药苷,心、肝、脾中均能检测到芍药苷,肺中芍药苷质量分数分别为0.076 4,0.042 0μg·g-1,肾中芍药苷质量分数分别为0.335,0.210μg·g-1;血瘀小鼠灌胃单缺牛膝方、缺桔梗和牛膝方后,脑、心、肝、脾中均几乎未检测到芍药苷,肺中芍药苷质量分数分别为0.274 7,0.019 7μg·g-1,肾中芍药苷质量分数分别为0.467 1,0.149 0μg·g-1。结论:基于缺桔梗和牛膝方比较,桔梗能增大肺、肾中芍药苷含量,对于血瘀小鼠,其增大肺中芍药苷含量的作用尤其显著;牛膝能减小正常小鼠肺、肾中芍药苷含量,能增大血瘀小鼠肺、肾中芍药苷含量,并使得芍药苷在心、肝、脾中分布。证实桔梗、牛膝载药至特定病所的的引经作用,且发现桔梗、牛膝的引经部位并不单一。
OBJECTIVE: To compare the effects of different combinations of Campanulaceae and Achyranthes bidentata on the distribution of paeoniflorin in normal and blood stasis mice, and to study the introduction of Campanulaceae and Achyranthes bidentata. Methods: Normal and blood stasis mice were fed with Xuefuzhuyu Decoction alone, single absence of bermudagrass, lack of Achyranthes bidentata, lack of bermudagrass and Achyranthes bidentata. After 30 minutes, the mice were sacrificed and the whole blood was collected. The brain, heart, liver, Spleen, lung, kidney. The content of paeoniflorin was determined by HPLC. The mobile phase was acetonitrile - water (16:84). The detection wavelength was 230 nm. Geniposide was used as internal standard. Results: Paeoniflorin was not detected in brain, heart, liver and spleen of normal mice after gavage of Xuefu Zhuyu Decoction, Paeoniflorin mass fraction were 0.010 2 0.005 8,0.091 0,0.037 3μg · g-1, respectively. The contents of paeoniflorin in kidney were 0.122 2 0,052 0,0.144 0,0.065 0 μg · g -1, respectively. Paeoniflorin could not be detected in the blood stasis mice, but paeoniflorin could be detected in the heart, liver and spleen. The mass fraction of paeoniflorin in lung was 0.076 4 , 0.042 0μg · g-1, and the content of paeoniflorin in kidney were 0.335,0.210μg · g-1 respectively. After blood-stasis mice were fed with single Achyranthes bidentata, Paeoniflorin was almost not detected in the spleen. The mass fraction of paeoniflorin in the lung was 0.274 7 and 0.019 7 μg · g -1, respectively, and the contents of paeoniflorin in the kidney were 0.467 and 0.1449 μg · g -1, respectively. Conclusion: Based on the lack of Campanulaceae and Achyranthes compared, Campanulaceae can increase lung and kidney paeoniflorin, for blood stasis mice, its role in increasing the content of paeoniflorin in the lung is particularly significant; Achyranthes can reduce the normal mouse lung , The content of paeoniflorin in kidney can increase the content of paeoniflorin in the lung and kidney of blood stasis mice and make the distribution of paeoniflorin in heart, liver and spleen. Confirmed Campanulaceae, Achyranthes drugs to a specific disease by the introduction of the role and found that Campanium, Achyranthes cited by the site is not single.