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利用表型性状和SSR标记对山西省41份青狗尾草资源进行遗传多样性分析。在表型水平,通过分析每份资源的株型、穗型、叶型等14个表型性状指标的测定结果,发现山西青狗尾草资源具有较为丰富的表型多样性,其中叶片绿色的深浅即叶绿素含量遗传多样性指数最高,为2.76,其余性状遗传多样性指数也大多保持在2.0左右;大多数青狗尾草的穗型为纺锤形,籽粒颜色和形状分别是青色和纺锤形。通过表型聚类可将山西青狗尾草资源划分为3个类群:第1类主要表现为株高、穗数等整体指标居中;第2类主要表现为植株小,穗数少,单株干重低等;第3类主要表现为植株高大,穗数多,穗重等。聚类分析发现表型多样性与山西特定生态地理区划有一定相关性。在分子水平,利用20对SSR引物共检测到102个等位变异,平均每个位点5个,基因多样性与PIC的平均值分别为0.66和0.61。虽然聚类分析、群体结构分析和主成分分析均将41份青狗尾草资源分为了3类,但3种方法对狗尾草资源的划分结果并不完全一致,且划分出的3个类群与山西的生态地理区划也不完全一致,表明不同表型和遗传背景的青狗尾草资源在山西混合分布,区域之间没有明显界限。
The genetic diversity of 41 Setaria viridis resources in Shanxi Province was analyzed by phenotypic traits and SSR markers. At the phenotypic level, the results of 14 phenotypic traits, such as plant type, panicle type, and leaf type, were analyzed. It was found that the resources of P. maxima in Shanxi were rich in phenotypic diversity. The green depth Chlorophyll content of the highest genetic diversity index of 2.76, the rest of the genetic diversity index are mostly maintained at about 2.0; most of green genie grass spike spindle type, grain color and shape were cyan and spindle shape. Through the phenotypic clustering, the genus of Shanxi green foxtail can be divided into three groups: the first class mainly showed the overall index of plant height and spike number, etc. The second group mainly consisted of small plant, fewer spikes, dry weight per plant Low; the third category mainly for tall plants, spike number, spike weight and so on. Cluster analysis found that the phenotypic diversity and Shanxi specific eco-geographical division have some relevance. At the molecular level, 102 SSR primers were used to detect 102 alleles, with an average of 5 loci per locus. The average value of genetic diversity and PIC was 0.66 and 0.61, respectively. Although cluster analysis, population structure analysis and principal component analysis divided 41 genus Green Gypsophila resources into three categories, the results of the three methods were not completely consistent with that of Setaria viridis, and the three groups were different from those of Shanxi Geographical zoning is also not exactly the same, indicating that the genus Green Setaria with different phenotypes and genetic backgrounds are mixed and distributed in Shanxi Province with no obvious boundaries between the regions.