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为观察脑缺血后神经元特异性烯醇化酶 (NSE)活性的动态变化以及高压氧 (HBO)治疗对其的影响 ,并探讨高压氧的治疗机制 ,将SD大鼠随机分为缺血再灌注组 (I/R)、高压氧处理组 (HBO)及假手术组。以四动脉阻断法建立脑缺血再灌注动物模型 ,缺血 2 0min。分别于再灌注 6、2 4、4 8及 96h取血 ,用酶联免疫吸附法 (ELISA)测定血浆中的NSE活性。结果显示各I/R组血浆NSE活性较假手术组均有升高 ,其中 6h及 96hI/R组与假手术组相比差异有显著性意义 (P <0 .0 5)。提示 :缺血再灌注损伤导致血浆NSE活性升高 ,I/R组的NSE活性出现 6h和 96h时的 2次升高 ,可能与脑缺血过程中出现的急性神经元坏死及其后的迟发性神经元凋亡相关。各HBO治疗组与相应时间点I/R组相比 ,6hHBO组的NSE活性较 6hI/R组显著降低 (P <0 .0 5) ,其他时间点HBO治疗组与相应I/R组相比无显著性差异 (P >0 .0 5)。提示 :HBO治疗作用表现为NSE活性的恢复比同时相点I/R组快 ,本研究中 6h为高压氧最佳治疗时窗
To observe the dynamic changes of neuron-specific enolase (NSE) activity and the effect of hyperbaric oxygen therapy (HBO) on cerebral ischemia, and to explore the mechanism of hyperbaric oxygen therapy, SD rats were randomly divided into ischemia and then Perfusion group (I / R), hyperbaric oxygen treatment group (HBO) and sham operation group. The animal model of cerebral ischemia-reperfusion was established by four-artery occlusion method, ischemia was 20 min. Blood samples were taken at 6, 2, 4, 8, 8 and 96 hours after reperfusion, respectively. NSE activity in plasma was measured by enzyme linked immunosorbent assay (ELISA). The results showed that plasma NSE activity in all I / R groups was higher than that in sham-operation group, and the difference was significant between 6h and 96hI / R group and sham operation group (P <0.05). It is suggested that the NSE activity of I / R group increased 2 times at 6h and 96h after ischemia-reperfusion injury, which may be associated with the acute neuronal necrosis and subsequent delayed Apoptosis Related to Neuronal Apoptosis. The NSE activity of 6hHBO group was significantly lower than that of 6hI / R group (P <0.05) at all HBO treatment groups compared with the corresponding time point I / R group. At other time points, HBO treatment group and corresponding I / R group No significant difference (P> 0.05). Hint: The therapeutic effect of HBO is that the recovery of NSE activity is faster than that of the I / R group at the same time. In this study, the optimal treatment time window of hyperbaric oxygen