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目的:建立参芪颗粒中人参皂苷Rg1,Re,Rb1的含量测定方法。方法:采用高效液相法对制剂中的人参皂苷Rg1,Re,Rb1进行含量测定。结果:人参皂苷Rg1在0.26～4.2μg线性关系良好,r=0.999 8,平均回收率99.4%,RSD 0.7%;人参皂苷Re在1.0～16.2μg线性关系良好,r=0.999 9,平均回收率99.6%,RSD 0.7%;人参皂苷Rb1在2.5～40.2μg线性关系良好,r=0.999 9,平均回收率99.2%,RSD 0.7%。结论:样品处理方法合理,方法学考察符合定量要求,结果准确,可用于参芪颗粒中人参皂苷Rg1,Re,Rb1的含量测定。
Objective: To establish a method for the determination of ginsenoside Rg1, Re, Rb1 in Shenqi granule. Methods: The content of ginsenoside Rg1, Re, Rb1 in the preparation was determined by HPLC. Results: Ginsenoside Rg1 had a good linear relationship at 0.26 ~ 4.2μg, r = 0.999 8, the average recovery was 99.4% and the RSD was 0.7%. The linear relationship of ginsenoside Re was 1.0 ~ 16.2μg, r = 0.999 9, the average recovery was 99.6 %, RSD 0.7%; ginsenoside Rb1 in 2.5 ~ 40.2μg good linear relationship, r = 0.999 9, the average recovery of 99.2%, RSD 0.7%. Conclusion: The sample processing method is reasonable and the methodological investigation meets the quantitative requirements. The results are accurate and can be used for the determination of ginsenoside Rg1, Re, Rb1 in Shenqi Granules.