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[Objective]This study aimed to optimize the in vitro regeneration system of melon.[Method]Melon variety Nanxiang 91023 was selected as experimental materials,with the cotyledons and hypocotyls as explants,different types and concentrations of growth regulators were supplemented at different stages of tissue culture,to explore the simple and effective medium formula for regeneration of melon.[Result]MS + 1.5 mg /L of 6-BA + 0.2 mg /L of IAA was the optimal medium for induction and proliferation of callus;MS + 1.0 mg /L of 6-BA + 0.2 mg /L of 2,4-D was the optimal medium for differentiation of adventitious buds;MS + 1.0 mg /L of ZT + 0.2 mg /L of IAA was the optimal medium for rooting of seedlings.On the basis of above conditions,melon seedlings had high rooting rate and strong roots.[Conclusion] This study provided a guarantee for the further genetic transformation of improved melon varieties.
[Objective] This study aimed to optimize the in vitro regeneration system of melon. [Method] Melon variety Nanxiang 91023 was selected as experimental materials, with the cotyledons and hypocotyls as explants, different types and concentrations of growth regulators were supplemented at different stages of [Result] MS + 1.5 mg / L of 6-BA + 0.2 mg / L of IAA was the optimal medium for induction and proliferation of callus; MS + 1.0 mg / L of 6-BA + 0.2 mg / L of 2,4-D was the optimal medium for differentiation of adventitious buds; MS + 1.0 mg / L of ZT + 0.2 mg / L of IAA was the optimal medium for rooting of seedlings.On the basis of above conditions, melon seedlings had high rooting rate and strong roots. [Conclusion] This study provided a guarantee for the further genetic transformation of improved melon varieties.