论文部分内容阅读
目的 :探讨益气养阴活血化瘀中药对微小残留白血病小鼠的作用及其凋亡机制。方法 :建立微小残留白血病模型 ,胃饲益气养阴活血化瘀中药 ,每次0 2ml,每日2次 ,观察生存期、外周血白细胞和血小板计数、脾细胞凋亡比例和细胞周期的变化。结果 :中药组生存期较模型组明显延长 ,P<0.01。外周血白细胞计数在治疗后第7天 ,中药组接近正常组水平 ,模型组显著高于正常组水平 ,P<0.01。中药组脾细胞凋亡比例显著高于模型组 ,P<0.01 ,模型组动物G2 +M期细胞有阻滞现象。结论 :益气养阴活血化瘀中药可通过诱导白血病细胞凋亡 ,延缓微小残留白血病的复发
Objective : To investigate the effect of Yiqi Yangyin Huoxuehuayu medicine on mice with minimal residual leukemia and its mechanism of apoptosis. METHODS: A minimal residual leukemia model was established. The stomach was fed with Qi and Yin, promoting blood circulation and removing blood stasis Chinese herbs, each time 0 2 ml, 2 times a day. The survival time, peripheral blood leukocyte and platelet counts, apoptosis ratio of splenocytes and cell cycle were observed. . Results: The survival time of the Chinese medicine group was significantly longer than that of the model group, P<0.01. The white blood cell count in peripheral blood was close to the normal group on the 7th day after treatment. The model group was significantly higher than the normal group, P<0.01. The proportion of apoptosis in spleen cells in the Chinese medicine group was significantly higher than that in the model group (P<0.01). The G2 + M phase cells in the model group had a block phenomenon. Conclusion : Yiqi Yangyin Huoxue Huayu traditional Chinese medicine can induce apoptosis of leukemia cells and delay the recurrence of minimal residual leukemia