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AIM:To study the loss of heterozygosity(LOH)on12p12-13 in Chinese colon carcinoma patients.METHODS:DNA was extracted from 10 specimens ofcancer tissue,10 specimens of adjacent tissue and 10specimens of normal tissue,respectively.LOH of Kras2gene was analyzed by polymerase chain reaction(PCR)and denaturing polyacrylamide gel electrophoresis using11 microsatellite markers on 12p-12-13.RESULTS:LOH of Kras gene was detected at least onone marker of 12p-12-13 in 30%(3/10)of adjacenttissue specimens.The highest frequency of LOH wasidentified on D12S1034 in 28.57%(2/7)of adjacenttissue specimens.LOH was detected at least on onemarker of 12p12-13 in 60%(6/10)of carcinoma tissuespecimens,the most frequent LOH was found onD12S1034 and D12S1591 in 42.86%(3/7)of carcinomatissue specimens.LOH was detected in 30%(3/10)ofcarcinoma tissue specimens,30%(3/10)of adjacenttissue specimens,and no signal in 1%(1/0)carcinomatissue specimen.The occurrence of LOH did not correlatewith sex,age,tumor size and lymph node metastasis.CONCLUSION:Genomic instability may occur on12p-12-13 of Kras2 gene in the development andprogression of colon carcinoma.The high LOH ofKras2 gene may directly influence the transcription andtranslation of wild type Kras2 gene.
AIM: To study the loss of heterozygosity (LOH) on12p12-13 in Chinese colon carcinoma patients. METHODS: DNA was extracted from 10 specimens of cancer tissue, 10 specimens of adjacent tissues and 10specimens of normal tissue, respectively. LOH of Kras2gene was analyzed by polymerase chain reaction (PCR) and denaturing polyacrylamide gel electrophoresis using11 microsatellite markers on 12p-12-13 .RESULTS: LOH of Kras gene was detected at least onone marker of 12p-12-13 in 30% (3/10) of adjacenttissue specimens The highest frequency of LOH was identified on D12S1034 in 28.57% (2/7) of adjacent tonsissues. LH was detected at least on one marker of 12p12-13 in 60% (6/10) of carcinoma tissuespecimens, the most frequent LOH was found (3/10) of adjacent tissue specimens, and no signal in 1% (1/2) were found in 30% (3/10) of carcinomatissue specimens. 0) carcinomatissue specimen. The occurrence of LOH did not correlatewith sex, age, tumor size and ly mph node metastasis. CONCLUSION: Genomic instability may occur on 12p-12-13 of Kras2 gene in the development andprogression of colon carcinoma. The high LOH of Kras2 gene may directly influence the transcription and translation of wild type Kras2 gene.