论文部分内容阅读
寻找一种快速、敏感、特异的诊断肾病综合征出血热 (HFRS)方法。方法 采用碘化钠 -异硫氰酸胍 -氯仿法抽提病毒核酸 ,逆转录聚合酶链反应 (RT-PCR)检测不同病程中 HFRS患者血清中的汉坦病毒感染情况 ,并采用间接酶联免疫吸附试验 (EL ISA)检测特异性 Ig M抗体。结果 84.7%患者血清中汉坦病毒核酸检测为阳性 ,其中病程为 1周内者检出率为 10 0 % ,1～ 2周者为 6 0 % ,超过 2周者为 33.3% ,而特异性 Ig M的总检出率为 70 .0 5 % ,病程在 1周、1～ 2周、超过 2周者检出率分别为 6 4%、90 %、6 6 .6 %。结论 RT-PCR和 ELISA都是 HFRS的特异性诊断方法 ,两者可相互补充 ,提高诊断率
To find a fast, sensitive and specific method for the diagnosis of hemorrhagic fever with nephrotic syndrome (HFRS). Methods The virus nucleic acid was extracted by sodium iodide-guanidinium isothiocyanate-chloroform method and the Hantaan virus infection in serum of patients with HFRS at different stages was detected by reverse transcription-polymerase chain reaction (RT-PCR) Immunosorbent assay (EL ISA) detects specific Ig M antibodies. Results The positive rate of hantavirus in serum was 84.7%. The detection rate was 100% in 1 week, 60% in 1 ~ 2 weeks and 33.3% in 2 weeks, while the specificity The overall detection rate of IgM was 70.0%. The detection rate of IgM was 64%, 90%, 66.6% at 1 week, 1 ~ 2 weeks and more than 2 weeks respectively. Conclusion Both RT-PCR and ELISA are specific diagnostic methods for HFRS, and they can complement each other to improve the diagnostic rate