论文部分内容阅读
通过水热法合成了石墨烯-金纳米复合材料。透射电子显微镜直接证明了制备的石墨烯薄而透明的片状结构。X-射线光电子能谱和X-射线衍射结果也表明了金的存在和氧化石墨烯的还原。作为一个模型,将肌红蛋白固定到该复合材料修饰的玻碳电极上并用于生物传感器。所固定的肌红蛋白显示了一对对称的氧化还原峰并对过氧化氢的还原具有高的催化活性。在信噪比等于3的时侯该生物传感器的线性范围在0.1到1.5μmol.L-1,检测限为0.05μmol.L-1,并且具有好的选择性、重现性和稳定性。
Graphene - Au nanocomposites were synthesized by hydrothermal method. Transmission electron microscopy directly proved that the prepared graphene thin and transparent sheet structure. X-ray photoelectron spectroscopy and X-ray diffraction results also show the presence of gold and the reduction of graphene oxide. As a model, myoglobin is immobilized on the composite modified glassy carbon electrode and used in biosensors. The immobilized myoglobin shows a pair of symmetrical redox peaks and has a high catalytic activity for the reduction of hydrogen peroxide. The biosensor has a linear range of 0.1 to 1.5 μmol·L-1 and a detection limit of 0.05 μmol·L-1 at a signal-to-noise ratio of 3 and has good selectivity, reproducibility and stability.