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目的:研究肠润通煎剂对链脲佐菌素所诱导的糖尿病大鼠结肠重构的影响。方法:40只SD雄性大鼠,30只大鼠造模后随机分为糖尿病对照组(DM组),肠润通高剂量治疗组(T1组)和肠润通低剂量治疗组(T2组),另外10只作为正常对照组(Con组)。肠润通治疗组经灌胃给药,定期测量大鼠体质量。实验结束时取出结肠中段,0.9%氯化钠溶液冲洗称湿重后,切取3个1-2mm厚的肠环用作无载荷和零应力状态测量,剩余肠段用作充压(从0-20cm水柱)实验。形态几何数据,展开角和残余应变可从无载荷和零应力状态测量中获得,结合充压肠管的压力和直径的变化,计算肠管的周向和纵向的应力和应变。结果:与Con组比较,DM,T1和T2组体质量显著降低(P<0.01)。与Con组比较,DM组的结肠肠管单位长度湿重体重比,壁厚,壁横截面积和展开角均明显增高(P<0.01),结肠壁的内外残余应变绝对值及周向和纵向刚度也明显增加(P<0.05)。T1处理能逆转糖尿病所致的形态学和生物力学变化(P<0.05),但T2处理后各项指标与DM组无显著差异。结论:肠润通煎剂(高剂量)治疗可以改善糖尿病所致的结肠形态学和生物力学重构。肠润通煎剂可能是通过改善肠道生物力学特性的重构而发挥治疗便秘的作用。
Objective: To study the effect of Changrun Tong decoction on colon reorganization induced by streptozotocin in diabetic rats. Methods: Forty male Sprague Dawley rats and 30 rats were randomly divided into three groups: diabetic control group (DM group), Changrun Tong high dose treatment group (T1 group) and Changrun Tong low dose treatment group (T2 group) , The other 10 as a normal control group (Con group). Intestinal Run Tong treatment group by gavage, regular measurement of body weight in rats. At the end of the experiment, the middle part of the colon was taken out and rinsed with 0.9% sodium chloride solution, then weighed and wet weight removed. Three 1-2mm thick intestine rings were cut out for no-load and zero-stress measurement, 20cm water column) experiment. Morphometric data, unfolding angle and residual strain can be obtained from no-load and zero-stress measurements. The circumferential and longitudinal stress and strain of the intestine are calculated in combination with changes in pressure and diameter of the compressed intestine. Results: Compared with Con group, the body weight in DM, T1 and T2 groups decreased significantly (P <0.01). Compared with the Con group, the DM group had a significant increase in wet weight and body weight ratio, wall thickness, wall cross-sectional area and unfolding angle (P <0.01), as well as the residual internal and external residual strain and the circumferential and longitudinal stiffness Also significantly increased (P <0.05). T1 treatment could reverse the morphological and biomechanical changes caused by diabetes (P <0.05), but there was no significant difference between T2 treatment and DM group. Conclusion: Changrun Tong decoction (high dose) treatment can improve diabetic colon morphology and biomechanical remodeling. Changrun Tong decoction may play a role in treating constipation by improving the reconstruction of intestinal biomechanics.