AMF增强枳抗旱性作用机制的初步研究

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丛枝菌根真菌(AMF)能增强寄主植物的抗旱性,但相关机制仍不清楚。本研究分析了正常水分和干旱处理对比下AM真菌Diversispora versiformis对盆栽枳(Poncirus trifoliata)生长、光合作用和叶片激素含量的影响。结果表明,6周的干旱处理强烈抑制D.versiformis对枳根系的侵染。干旱处理下,AM真菌接种处理均显著提高了叶、茎和根系生物量,显著增强了叶片光合速率、蒸腾速率和气孔导度,同时显著降低了叶片温度(P<0.05)。AM真菌接种也增加了干旱条件下枳叶片内源脱落酸、吲哚乙酸、赤霉素、油菜素内酯、茉莉酸甲酯、玉米素核苷和一氧化氮含量。推测AM真菌通过改变植物内源激素平衡状况,进而诱导相关激素信号传导进程,从而参与菌根的形成、植物生长进程,并增强植物抗旱性。 Arbuscular mycorrhizal fungi (AMF) can enhance the drought resistance of host plants, but the mechanism remains unclear. This study analyzed the effects of AM fungi Diversispora versiformis on the growth, photosynthesis and leaf hormone contents of Poncirus trifoliata under normal conditions of water stress and drought stress. The results showed that 6-week drought treatment strongly inhibited D. versiformis’s infection of trifoliate orange root. Under drought stress, AM fungal inoculation significantly increased leaf, stem and root biomass, significantly increased leaf photosynthetic rate, transpiration rate and stomatal conductance, and significantly decreased leaf temperature (P <0.05). Inoculation with AM fungi also increased the content of endogenous ABA, indoleacetic acid, gibberellin, brassinosteroid, methyl jasmonate, zeatin riboside and nitric oxide in leaves under drought conditions. It is speculated that AM fungi may participate in the formation of mycorrhizae, the process of plant growth and enhance the drought resistance of plants by altering the balance of endogenous hormones in plants and thereby inducing the related hormonal signaling processes.
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