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人可通过职业和非职业方式接触镉和镍。研究表明,肝脏是这两种金属的主要靶器官,并可影响肝单加氧酶(即细胞色素P—450)活性。已知,在生产镉—镍电池时,人可同时接触这两种金属。本研究的目的,在于观察镉和镍对大鼠肝内某些细胞色素P—450同功酶活性的联合作用。第1组雄性大鼠在处死前72h,经腹腔注射给镉(CdCl_2·6H_2O)3.58mg/kg体重。第2组雄性大鼠在处死前16h,经皮注射镍(NiC_(12)·6 H_2O)59.5mg/kg体重。以上所给镉与镍的剂量,为该两种金属分别引起单加氧酶酶活性最大抑制的剂量,出现乙基吗啡—N—脱甲基酶、氨基吡啉—N—脱甲基酶和苯胺—4—羟化酶的活性显著下降(P<0.05);细胞色素P—450
People can access cadmium and nickel both professionally and non-professionally. Studies have shown that the liver is the major target organ for both metals and can affect hepatic monooxygenase (ie, cytochrome P-450) activity. It is known that in the production of a cadmium-nickel battery, one can contact both metals at the same time. The purpose of this study was to investigate the combined effects of cadmium and nickel on the activity of some cytochrome P-450 isoenzymes in rat livers. The male rats in group 1 were given intraperitoneal injection of cadmium (CdCl 2 · 6H 2 O) 3.58 mg / kg body weight 72h prior to sacrifice. The male rats in group 2 were injected with 59.5 mg / kg body weight of nickel (NiC_ (12) .6H_2O) 16 h before sacrifice. The doses of cadmium and nickel given above are the doses at which the two metals cause the greatest inhibition of monooxygenase activity, respectively. Ethylmorpholine-N-demethylase, aminopyrine-N-demethylase and Aniline-4-hydroxylase activity was significantly decreased (P <0.05); cytochrome P-450