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目的:建立老年咳喘片(薄膜衣片)薄层鉴别及含量测定方法。方法:采用TLC对补骨脂中补骨脂素、异补骨脂素,淫羊藿中淫羊藿苷进行定性鉴别;采用HPLC-ELSD测定了黄芪中黄芪甲苷的含量。结果:在0.9669～6.446μg,黄芪甲苷峰面积常用对数值与进样量常用对数值呈良好的线性关系,r=0.9998,平均回收率为96.5%,RSD为1.05%。结论:薄层色谱法呈现斑点清晰,专属性强;HPLC-ELSD简便准确,重复性好,可有效控制老年咳喘片(薄膜衣片)的质量。
Objective: To establish a method for the identification and determination of the content of cough asthma tablets (film-coated tablets). Methods: TLC was used to qualitatively identify icariin in psoralen, isopsoralen and icariin. The content of astragaloside Ⅳ in Radix Astragali was determined by HPLC-ELSD. Results: In the range of 0.9669 ~ 6.446μg, the logarithm of astragaloside peak area showed a good linear relationship with the common logarithm of injection volume, r = 0.9998, the average recovery was 96.5% and the RSD was 1.05%. Conclusion: Thin-layer chromatography (TLC) shows clear spots and high specificity. HPLC-ELSD is simple, accurate and reproducible, which can effectively control the quality of the tablets.