截短型H1N1神经氨酸酶基因的克隆及其在毕赤酵母中的表达

来源 :中国医药工业杂志 | 被引量 : 0次 | 上传用户：comboyaoqiu

【摘要】

：

将密码子优化的截短型H1N1神经氨酸酶基因克隆到载体pPICZαA中,以构建重组质粒pPICZαA-NAS.将线性化的重组质粒pPICZαA-NAS转化毕赤酵母SMD1168,获得了能够分泌表达截短型

【作者】

：

林军许明飞胡海峰胡又佳朱宝泉

【机构】

：

中国医药工业研究总院上海医药工业研究院

【出处】

：

中国医药工业杂志

【发表日期】

：

2004年期

【关键词】

：

H1N1 神经氨酸酶基因克隆表达

【基金项目】

：

国家重大新药创制科技重大专项资助;第47批中国博士后科学基金

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将密码子优化的截短型H1N1神经氨酸酶基因克隆到载体pPICZαA中,以构建重组质粒pPICZαA-NAS.将线性化的重组质粒pPICZαA-NAS转化毕赤酵母SMD1168,获得了能够分泌表达截短型H1N1神经氨酸酶的重组菌株.结果表明,重组菌株能够分泌表达具有生物活性的截短型神经氨酸酶,该酶受两种已知的神经氨酸酶抑制剂(奥司他韦和扎那米韦)的显著抑制,可用于构建新型神经氨酸酶抑制剂的高通量筛选模型.

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截短型H1N1神经氨酸酶基因的克隆及其在毕赤酵母中的表达

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