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比较几种烟碱型乙酰胆碱受体(nAChR)激动剂及拮抗剂对颈上神经节(SCG)培养神经元nAChR的作用和SCG培养神经元nAChR的药理特征.方法:用全细胞膜片钳技术结合局部压力喷射给药观察培养的新生大鼠SCG神经元对胆碱能药物的反应.结果:所测试的交感神经元均可被nAChR激动剂所激动,激动剂在相同浓度下诱发的电流峰值分别为:乙酰胆碱(ACh),443±183 pA;烟碱,1175±377 pA;碘化1,1-二甲基-4-苯基哌嗪(DMPP),2946±358 pA.在几种nAChR拮抗剂中,美加明(Mec),六烃季胺,箭毒在相同浓度下拮抗DMPP引起的全细胞电流的峰值分别为:435±154 pA,725±320 pA,887±214 pA,但α-银环蛇毒对之无影响.Mec对DMPP电流的拮抗具有使用依赖性.当Mec 1 μmol·L~(-1)与DMPP 100 μmol·L~(-1)混合向SCG神经元压力喷射时,前6次电流分别为第一次的％:100,64±3,50±3,41±4,36±4,32±3％.结论:SCG神经元的nAChR与骨胳肌及中枢nAChR有不同的药理特征.
To compare the effect of nAChR agonists and antagonists on nAChR in cultured SCG neurons and the pharmacological characteristics of nAChR in cultured SCG neurons.Methods: Whole-cell patch-clamp technique Local pressure jet administration was used to observe the response of cultured SCG neurons to cholinergic drugs.Results: The tested sympathetic neurons were all excited by nAChR agonists, and the peak currents induced by agonists at the same concentration were (ACh), 443 ± 183 pA, nicotine, 1175 ± 377 pA, 1,1-dimethyl-4-phenylpiperazine iodide (DMPP), 2946 ± 358 pA. In several nAChR antagonists (Mec), hexaalkylamine and arrowroot, respectively, the peak values of total cell current induced by DMPP were 435 ± 154 pA, 725 ± 320 pA and 887 ± 214 pA at the same concentration, Bungarus snake venom had no effect on it.The antagonism of Mec to DMPP current was dependent.When Mec 1 μmol·L -1 mixed with 100 μmol·L -1 DMPP injected into SCG neurons, The first six currents were the first%: 100,64 ± 3,50 ± 3,41 ± 4,36 ± 4,32 ± 3% .Conclusion: The nAChR of SCG neurons and the skeletal muscle and central nAChR are Different pharmacology feature.