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采用滤纸片琼脂平板扩散法和微量肉汤稀释法检测了锈苞蒿挥发油对2种真菌和10种细菌(包括5种临床致病菌)的体外抑菌杀菌活性,结果显示,挥发油对大部分微生物均具有很好的抗菌作用,尤其对酵母菌、革兰氏阳性菌的抗菌活性更强,其最低抑菌浓度(minimal inhibitory concentration,MIC)为0.02~0.31 g/L,最低杀菌浓度(minimum bactericidal concentration,MBC)为0.04~1.25 g/L.其次利用气相色谱-质谱法(GC-MS)技术分析了锈苞蒿挥发油的化学组成,确定了28种成分的化学结构与相对含量,约占挥发油总组分的81.41%,主要由单萜和倍半萜组成,其中Vulgarnoe B(26.58%)、1,8-桉叶素(19.89%)和樟脑(7.91%)是主要成分.
The in vitro antibacterial and bactericidal activity of Artemisia selengensis volatile oil against 2 fungi and 10 bacteria (including 5 clinical pathogenic bacteria) was tested by filter paper agar plate diffusion method and trace broth dilution method. Microorganisms have very good antibacterial activity, especially against yeasts and Gram-positive bacteria, their minimum inhibitory concentration (MIC) is 0.02~0.31 g/L, the minimum bactericidal concentration (minimum The bactericidal concentration (MBC) was 0.04~1.25 g/L. The chemical composition of volatile oil of Artemisia selengensis was analyzed by gas chromatography-mass spectrometry (GC-MS) technique. The chemical structures and relative contents of 28 components were determined, accounting for approximately 81.41% of the total volatile oil components, mainly composed of monoterpenes and sesquiterpenes, of which Vulgarnoe B (26.58%), 1,8-cineole (19.89%) and camphor (7.91%) are the main components.