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目的研究选择性COX-2抑制剂塞来昔布对癫痫大鼠的保护作用。方法采用海马内注射海人藻酸(Kainic Acid,KA,1μg/μL,1.0μL)建立大鼠癫痫模型,观察并记录各组大鼠注射KA后的行为学变化;采用免疫组化法检测大鼠海马组织中离子钙接头蛋白分子(Iba-1)和胶质纤维酸性蛋白(GFAP)的表达情况。结果塞来昔布组的癫痫发作潜伏期明显延长,最大发作级别明显降低(P<0.05);与模型组相比,塞来昔布组的海马组织中Iba-1和GFAP的表达减少,胶质细胞活化情况明显减轻。结论塞来昔布可能通过抑制小胶质细胞和星形胶质细胞活化,减轻其介导的炎症反应,改善癫痫发作的程度。
Objective To study the protective effect of selective COX-2 inhibitor celecoxib on epileptic rats. Methods Kainic acid (KA, 1μg / μL, 1.0μL) was injected intraperitoneally into the hippocampus to establish the rat epilepsy model. The behavioral changes of rats in each group were observed and recorded after KA injection. Immunohistochemistry Expression of Iba-1 and GFAP in hippocampus of rats. Results Compared with the model group, the expression of Iba-1 and GFAP in the celecoxib group was significantly lower than that in the model group (P <0.05). The latency of seizure in the celecoxib group was significantly prolonged and the maximum seizure level was significantly decreased Cell activation was significantly reduced. Conclusion Celecoxib may reduce the activation of microglia and astrocytes, relieve the inflammatory reaction and improve the degree of seizure.