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杂色曲霉素是我国肿瘤高发区粮食中的优势污染霉菌毒素。为探讨杂色曲霉素 (ST)对人体免疫机能的影响 ,采用双抗体夹心ELISA方法对ST作用后人外周血单核细胞 (HPBMc)培养上清液中白血细胞介素Ⅱ (IL 2 )的分泌水平进行了检测。结果表明 ,不同浓度 ( 0 0 3 12 5～ 2mg L)ST处理 2 4h后 ,体外培养的HPB Mc的IL 2分泌均受到一定程度的抑制 ,其中以较低浓度ST( 0 0 3 12 5～ 0 12 5mg L)和较高浓度ST( 1～ 2mg L)抑制作用最明显 (P <0 0 5 )。在ST 1mg L作用 1～ 64h的时间范围内 ,ST对HPBMcIL 2的分泌总体表现抑制作用。ST处理后 8～ 64h ,随ST处理时间的延长 ,对IL 2分泌的抑制作用逐渐增强 (r =0 82 2 ,P <0 0 5 )。本研究结果提示 ,ST对HPBMcIL 2的分泌有一定的抑制作用
Ascorbic acid is the predominant contaminating mycotoxin in grain in the high incidence of cancer in our country. To investigate the effect of streptozotocin (ST) on human immune function, interleukin-2 (IL-2β) in supernatant of human peripheral blood mononuclear cells (HPBMc) The level of secretion was tested. The results showed that IL 2 secretion of HPB Mc cultured in vitro was inhibited to a certain extent after treated with different concentrations of ST (0 0 3 12 5 ~ 2 mg L) for 24 h, with a lower concentration of ST (0 0 3 12 5 ~ 0 12 5mg L) and higher concentrations of ST (1-2mg L) the most obvious inhibition (P <0 05). In the time range of 1 ~ 64h ST 1mg L, ST inhibited the secretion of HPBMcIL 2 in general. After ST treatment, the inhibitory effect of IL 2 secretion gradually increased with the increase of ST treatment time (r = 0 82 2, P 0 05). The results of this study suggest that ST inhibits the secretion of HPBMcIL 2 to a certain extent