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为探讨黄芩对短期干旱复水的生理生态响应。测定分析不同水分处理黄芩短期干旱复水期间叶片光合作用和叶绿素荧光参数、根中保护酶及黄酮合成关键酶活性等生理生化表征指标及根中黄芩苷、黄芩素积累的动态变化。结果显示,随干旱加剧各处理黄芩P n,T r,G s,F v/F m下降,F o升高;处理Ⅰ,Ⅱ黄芩根中SOD,POD活性对干旱响应比处理Ⅲ黄芩早;各处理黄芩的黄芩苷积累响应时间和增加幅度存在差异。复水后黄芩各指标有所恢复。因此,干旱导致土壤含水量降低,黄芩光合作用发生改变,破坏了抗氧化代谢平衡。黄芩启动自身防御机制,SOD,POD等保护酶,PAL,C4H等黄酮类代谢关键酶以及黄芩苷,黄芩素等黄酮类化合物协同作用,减少活性氧对细胞的伤害。
To explore the physiological and ecological response of Scutellaria baicalensis Georgi to short-term drought and rewatering. The changes of photosynthesis and chlorophyll fluorescence parameters, the activities of protective enzymes in the roots and the activities of key enzymes involved in flavonoid synthesis and the accumulation of baicalin and baicalein in the roots of S.sorachis under different water treatments were measured and analyzed. The results showed that the P n, T r, G s, F v / F m and F o of Scutellaria baicalensis Georgi increased with the increase of drought stress. Scutellaria baicalin treatment of baicalin accumulation response time and increase in the difference. Scutellaria after rehydration various indicators have been restored. Therefore, the drought caused the decrease of soil water content and the change of photosynthesis of Scutellaria baicalensis Georgi, which destroyed the balance of antioxidant metabolism. Scutellariae initiates its own defense mechanism, SOD, POD and other protective enzymes, PAL, C4H and other key enzymes of flavonoid metabolism and flavonoids such as baicalin, baicalein and synergistic effect, reduce reactive oxygen species damage to cells.