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目的比较4种HE染色方法制作旋毛虫肌幼虫囊包标本的效果。方法 HE染色方法1:苏木精染色5min,伊红染色5min,分色;HE染色方法2:苏木精染色15min,伊红染色5min,分色;HE染色方法3:苏木精染色1h,伊红染色30min,分色;HE染色方法4:苏木精染色1h,伊红染色1h,分色。结果 HE染色方法4制作的旋毛虫肌幼虫囊包标本,囊包梭形显著,结构清晰,囊内幼虫特征典型。结论 HE染色方法4制作的标本,染色效果好,易于观察。
Objective To compare the effect of four kinds of HE staining methods on the production of parasellar larvae. Methods HE staining method 1: hematoxylin staining 5min, eosin staining 5min, color separation; HE staining method 2: hematoxylin staining 15min, eosin staining 5min, color separation; HE staining method 3: hematoxylin staining 1h, Eosin staining 30min, color separation; HE staining method 4: hematoxylin staining 1h, Eosin staining 1h, color separation. Results Hematoxylin and eosinophil larvae were produced by HE staining. Conclusion HE staining method 4 produced specimens, staining effect is good, easy to observe.