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目的应用和验证快速鉴别麻疹疫苗株与野毒株病毒的方法。方法采用逆转录-聚合酶链反应-限制性片段长度多态性分析(Reverse Transcription-Polymerase Chain Reaction-Restriction Fragment Length Polymorphism,RT-PCR-RFLP)方法,对四川省2009～2011年分离的麻疹疫苗株和野毒株病毒进行鉴定,同时与序列分析进行对比验证。结果 5株RT-PCR扩增阳性产物,经AflⅡ酶切后,1株麻疹疫苗株病毒被切成两个片段,分别为287碱基对(Base Pair,bp)和151bp;4株麻疹野毒株病毒均不能被AflⅡ酶切,仍呈单一条带。结论 RT-PCR-RFLP是一种快速、简便的鉴别麻疹疫苗株与野毒株病毒的方法。
Objective To apply and validate a rapid method for the identification of measles and wild-type viruses. Methods Reverse transcriptase-polymerase chain reaction-restriction fragment length polymorphism (RT-PCR-RFLP) was used to detect the measles vaccine isolated in Sichuan Province from 2009 to 2011 Strains and wild-type virus were identified, at the same time compared with the sequence analysis to verify. Results Five positive products were amplified by RT-PCR. After digestion with Afl Ⅱ, one strain of measles vaccine strain was cut into two fragments, which were 287 bp and 151 bp, respectively. Four measles virus Strain virus can not be Afl Ⅱ digestion, was still a single band. Conclusion RT-PCR-RFLP is a rapid and simple method for the identification of measles vaccine strains and wild-type viruses.