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目的目的探讨大鼠高浓度矽尘接触过程中是否存在氧化应激反应。方法选40只Wistar雄性大鼠随机分为4组,即高剂量染尘组(1000 mg/m3)、中剂量染尘组(500 mg/m3)、低剂量染尘组(100 mg/m3)和对照组,选用动式染尘装置每天染尘2小时。染尘49天后处死大鼠,测定血清超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、总抗氧化能力(T-AOC)活性及丙二醛(MDA)、还原性谷胱甘肽(GSH)含量;取肺组织HE染色光镜下观察病理形态的改变。结果长时间、高浓度矽尘接触降低血清SOD、TAC、GSH、T-AOC水平,同时MDA含量升高,与对照组比较,差异有统计学意义(P<0.05),病理学检查出现炎症性特征变化。结论大鼠高浓度矽尘接触过程中体内可能存在氧化系统与抗氧化系统的失衡,氧化应激反应可能是矽尘接触早期症状之一。
Objective To investigate the oxidative stress in rats exposed to high concentrations of silica dust. Methods Forty male Wistar rats were randomly divided into four groups: high dose (500 mg / m3), low dose (100 mg / m3) And the control group, the choice of moving dust device dust daily 2 hours. The rats were killed after 49 days and the activities of superoxide dismutase (SOD), catalase (CAT), total antioxidant capacity (T-AOC) and malondialdehyde (MDA) Peptide (GSH) content was measured. Pathological changes of lung tissue were observed under light microscope. Results Compared with the control group, the exposure to high concentrations of silica dust for a long time reduced the levels of serum SOD, TAC, GSH and T-AOC, and increased the content of MDA. The difference was statistically significant (P <0.05) Feature changes. Conclusion There may be an imbalance of oxidation system and antioxidant system in the process of exposure to high concentrations of silica dust in rats. Oxidative stress may be one of the early symptoms of silica dust exposure.