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采用人肝微粒体温育实验 ,根据 CYP45 0 1A2特异性抑制法及 HPL C机电化学分析仪检测结果 ,发现 :1催化 MT在肝脏羟化和去甲基反应的 CYP45 0异构酶确证是 CYP45 0 1A2。 2 CYP45 0 1A2催化 MT6位羟化反应的Km 是 (4.48± 1.49) μm ol/ L (x± s) ,Vmax是 (13.83± 2 .5 2 ) pm ol/ (m g protein· min) (x±s)。 3催化 MT5位上 O-去甲基主要是 CYP45 0 1A2 ,还有 2 C19参与 ,其 Km值分别为 0 .0 30 μmol/ L 和 8.796 μmol/ L,Vmax分别是 0 .338pm ol/(mg protein· min)和 3.16 7pm ol/ (mg protein·m in)
Using human liver microsomal incubation test, CYP45 0 1A2 specific inhibition method and HPL C electromechanical analyzer test results showed that: 1 catalyzed MT hydroxylation and demethylation reaction CYP45 0 isomerase confirmed CYP45 0 1A2. The Km of CYP45 0 1A2 catalyzed hydroxylation at MT6 was (4.48 ± 1.49) μmol / L (x ± s) and Vmax was (13.83 ± 2.52) pmol / (mg protein · min) ± s). 3 catalyzed the O-demethylation of MT5 at CYP45 0 1A2 and 2 C19, with Km values of 0. 30 μmol / L and 8.796 μmol / L, respectively, with Vmax of 0.338 pmol / (mg protein · min) and 3.16 7 pm ol / (mg protein · m in)