1.保证菌种的纯培养,注意不要用被杂菌污染的菌种,不要近距离相连接培养。2.严格控制菌种传代次数,减少机械损伤,保证菌种活力。3.在适当的温度保存菌种:低温型菌种如蘑菇、香菇等,在4℃有利于保存菌丝体活力;高温型菌种如草菇,在16℃有利于保存菌丝体活力。4.避免在单一培养基中多次传代;菌种不宜过长时间使用,超龄菌种会出现老化,而老化与退化是有机相连的,生活力弱的 1. To ensure pure culture of strains, be careful not to be contaminated with bacteria bacteria, do not close connection training. 2. Strict control of bacteria passage times, reduce mechanical damage, to ensure the vitality of bacteria. 3. Stored in the appropriate temperature strains: low temperature type mushrooms, mushrooms, etc., at 4 ℃ conducive to preservation of mycelium vitality; high temperature type mushrooms, such as straw mushroom, at 16 ℃ conducive to preservation of mycelium vitality. 4. Avoid multiple passages in a single medium; strains should not be used for too long, over-aging species will be aging, and aging and degradation are organic, weak vitality