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背景:联合检测对于早期诊断BK病毒相关性肾病有着重要意义,目前研究较少。目的:探讨肾移植后联合检测诊断BK病毒相关性肾病的方法,并初步分析其临床应用价值。方法:留取84例肾移植患者的尿液和外周血标本,进行BK病毒感染负荷的实时荧光定量PCR和尿液巴氏染色检测,同时进行移植肾病理穿刺病理组织学及免疫组化检测,用于早期诊断BK病毒相关性肾病。结果与结论:2例患者诊断为BK病毒相关性肾病,占移植肾活检病例的2.5%。2例患者证实BK病毒相关性肾病后予以免疫抑制剂减量,肾功能明显好转。结果显示以上方法联合检测可提高BK病毒相关性肾病的诊断率,简便、可靠、准确,为进一步研究BK病毒感染与肾移植后移植物丢失的关系奠定了基础。
BACKGROUND: Co-detection is of great importance for the early diagnosis of BK-associated nephropathy. At present, few studies have been done. Objective: To explore the method of combined detection of renal allograft after BK virus-associated nephropathy, and to analyze its clinical value. Methods: Urine and peripheral blood samples were collected from 84 renal transplant recipients. Real-time PCR and urine Pap test were performed for BK virus infection. Pathological examination and immunohistochemistry were performed. Used for early diagnosis of BK virus-associated nephropathy. RESULTS AND CONCLUSION: Two patients were diagnosed as BK virus-associated nephropathy, accounting for 2.5% of all cases. 2 patients confirmed BK virus-associated nephropathy to immunosuppressive agents to reduce renal function improved significantly. The results show that the above method combined detection can improve the diagnosis rate of BK virus-associated nephropathy, which is simple, reliable and accurate, which lays the foundation for further study on the relationship between BK virus infection and graft loss after renal transplantation.