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目的:从分子水平阐明情志致病的机制及药物干预作用。方法:采用逆转录多聚酶链反应(RT—PCR )的分子生物学技术,观察大鼠束缚浸水后下丘脑及胃粘膜中促肾上腺皮质激素释放因子信使核糖核酸(CRFmRNA)表达,及具有理气降逆散结作用的中药复方心身1号干预作用的分子生物机制。结果:与正常组比较,模型组大鼠呈现气机紊乱证表现、胃内可见广泛性浅表性溃疡,下丘脑及胃粘膜出现CRFmRNA表达,而服心身1号的中药组大鼠胃内溃疡显著减少,CRFmRNA表达量明显降低。结论:证实气机紊乱证与CRF基因表达相关,心身1号具有改善上述指标的作用。
Objective: To elucidate the mechanisms of emotional pathogenesis and drug interventions at the molecular level. METHODS: The molecular biological techniques of reverse transcription-polymerase chain reaction (RT-PCR) were used to observe the expression of corticotropin-releasing factor mRNA (CRFmRNA) in the hypothalamus and gastric mucosa after the rats were bound to soak in water, and to observe the down-regulation of CRF mRNA. Molecular biological mechanism of traditional Chinese medicine compound Xinshen No.1 intervention effect of scavenging effect. RESULTS: Compared with the normal group, rats in the model group presented signs of gastrointestinal disorder, extensive superficial ulcers were observed in the stomach, CRFmRNA expression was observed in the hypothalamus and gastric mucosa, and gastric ulcer was observed in the rats of the traditional Chinese medicine group of Fuxinshen No.1. Significantly reduced, CRFmRNA expression was significantly reduced. Conclusion: It is confirmed that the gastrointestinal disorder syndrome is related to the expression of CRF gene, and Xinshen 1 has the effect of improving the above index.